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. Author manuscript; available in PMC: 2016 Nov 19.
Published in final edited form as: Brain Res. 2015 Sep 26;1627:90–100. doi: 10.1016/j.brainres.2015.09.018

Figure 1. CSE preparation and endothelial viability assessment following antioxidant treatments.

Figure 1

Panel A) Concentrated Smoke Extract (CSE) preparation by a Single Cigarette Smoking Machine according to ISO/FTC protocol. (Puff volume- 35ml, duration-2s, interval-58s; 8 puffs/cigarette); Smoking protocol is computer controlled. Panel B) results from the lactate dehydrogenase (LDH) assay using the Pierce™ LDH cytotoxicity kit. No significant change in LDH release was observed in all exposure conditions in comparison to the control. Microscopic images of intact monolayers of hCMEC/D3 cells after treatments. n = 5 biological replicates.