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. Author manuscript; available in PMC: 2016 Nov 6.
Published in final edited form as: Circ Res. 2015 Sep 17;117(11):956–966. doi: 10.1161/CIRCRESAHA.115.306783

Figure 4. CSN8hypo aggravates CryABR120G-induced aberrant protein aggregation and decreases the ubiquitination in mouse hearts.

Figure 4

Littermate mice with the indicated genotypes were used at the age of 8 weeks. (A) Representative confocal fluorescent images of myocardial sections immunostained for CryAB (white). Scale bar = 20 µm. (B) Quantitative analysis of the relative abundance of the CryAB-positive protein aggregates in the heart. n=3 mice/group. *p<0.05 vs. CTL::CryABR120G, t-test. (C) Filter trapping assays for CryAB and ubiquitin (Ub) in ventricular myocardium of mice with indicated genotypes. The proteins retained on the filter were immunostained for CryAB or Ub. The relative density of each dot is shown below the blots. (D) Western blot analyses of myocardial total ubiquitinated proteins. β-tubulin was probed as a loading control.