Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Neurochem. 2015 Sep 22;135(4):727–741. doi: 10.1111/jnc.13349

Figure 5.

Figure 5

Activation of adenylyl cyclase with forskolin inhibits the activity of AMPK. The retinas were excised and cultured in DD after chick embryos were entrained under LD cycles for 7 days. On the second day of DD, the cultured retinas were treated with DMSO (0.1%), forskolin, or forskolin+AICAR for 2 hr prior to harvest for immunoblotting at CT 4 and CT 16. (A) Treatment with forskolin (20 μM) at CT 4 significantly decreased the phosphorylation of AMPK at Thr172 (pAMPK) compared to the control (0.1% DMSO). * indicates that pAMPK is significantly higher in the control at CT 4 than the other three groups. (B1 and B2) Treatment with forskolin alone significantly increases the L-VGCC current densities when cones were recorded during the day (ZT 4–8), and AICAR dampens the augmentation effect of forskolin on L-VGCCs. * p<0.05.