Table 4.
Behavioral effects of N. nucifera partitions, compounds 1, 7, and 5–7 using tetrad assay.
| Treatment (mg/Kg) |
Locomotor Activity |
Hotplate Latency (% MPE) |
Catalepsy Latency (Sec) |
Rectal temperature (°C) |
Tail-Flick Latency (% MPE)a |
|---|---|---|---|---|---|
| Basic CHCl3 partition | |||||
| 10 | 1538 ± 186.1 | 7.39 ± 4.08 | 3.13 ± 1.59 | −0.19 ± 0.08 | 9.44 ± 5.37 |
| 30 | 606.4 ± 127.0*** | 5.90 ± 4.87 | 1.0 ± 0.0 | −0.75 ± 0.19 | 15.35 ± 12.79 |
| 75 | 109.0 ± 109.0*** | 57.64 ± 11.80** | 16.38 ± 8.39 | −5.33 ± 0.44*** | 46.79 ± 13.84* |
| 100 | 69.25 ± 19.09*** | 74.05 ± 13.70*** | 13.75 ± 10.16 | −4.61 ± 0.31*** | 62.43 ± 15.53*** |
| Acidic CHCl3 partition | |||||
| 10 | 1315 ± 225.8 | 28.33 ± 6.48 | 1.50 ± 0.34 | −1.81 ± 0.43*** | 11.80 ± 10.49 |
| 30 | 165.3± 67.56*** | 9.44 ± 4.84 | 1.90 ± 0.41 | 0.11 ± 0.32 | 14.61 ± 7.819 |
| 75 | 91.3 ± 37.56*** | 89.46 ± 9.25*** | 10.20 ± 4.33 | −5.96 ± 0.24*** | 54.73 ± 14.99** |
| 100 b | 103.0 ± 33.81*** | 85.73 ± 9.19 *** | 71.60 ± 17.21*** | −7.2 ± 0.23*** | 70.05 ± 12.40*** |
| 5 – 7 mixture | |||||
| 10 | 1789 ± 163.9 | 10.58 ± 1.68 | 4.30 ± 1.43 | 0.50 ± 0.13 | 5.46 ± 4.72 |
| 30 | 1865 ± 131.0 | 13.33 ± 3.99 | 3.80 ± 1.63 | 0.62 ± 0.23 | −3.39 ± 6.89 |
| 75 | 137.8 ± 43.35*** | 14.83 ± 5.96 | 8.00 ± 3.81 | −2.49 ± 0.28*** | 22.09 ± 13.52 |
| 100 | 30.2 ± 7.318*** | 26.3 ± 6.57 | 7.89 ± 2.37 | −4.45 ± 0.24*** | 42.90 ± 15.68* |
| Coclaurine (7) | |||||
| 10 | 1678 ± 270.3 | 10.15 ± 4.45 | 1.25 ± 0.25 | 0.17 ± 0.08 | 16.57 ± 7.23 |
| 30 | 1798 ± 170.5 | 5.46 ± 5.05 | 2.37 ± 0.59* | 0.1 ± 0.15 | 1.43 ± 6.95 |
| 75 | 1132 ± 132.7 | 15.49 ± 3.57 | 1.75 ± 0.41 | −0.39 ± 0.05 | 13.95 ± 5.01 |
| 100 | 764.9 ± 194.0** | 6.08 ± 1.9 | 1.25 ± 0.25 | −0.58 ± 0.23* | 10.07 ± 3.31 |
| Nuciferine (1) | |||||
| 10 | 1318 ± 252.6 | 6.99 ± 2.61 | 3.875 ± 2.32 | −0.49 ± 0.31 | 11.69 ± 6.074 |
| 30 | 1022 ± 224.1 | 3.69 ± 3.01 | 1.750 ± 0.62 | −0.91 ± 0.31 | 18.43 ± 10.50 |
| 75 | 593.9 ± 268.2** | 0.68 ± 2.73 | 3.500 ± 1.07 | −1.68 ± 0.38** | 19.45 ± 6.16 |
| 100 | 165.1 ± 58.19*** | 21.78 ± 7.16 | 1.500 ± 0.27 | −3.8 ± 0.57*** | 14.14 ± 15.52 |
| Vehicles | |||||
| ETOH:Cre mophor:Sa line (1:1:18) | 1668 ± 109.5 | 10.81 ± 4.93 | 1.100 ± 0.10 | −0.09 ± 0.08 | −2.97 ± 7.66 |
a
MPE; % of maximum effect.
b
Acidic partition exhibited clonic-tonic seizures upon touch at a dose of 100 mg/kg. The following asterisks indicates the significances,
*
p<0.05,
**
p<0.01,
***
p<0.001 versus the vehicle, Dunnett’s post hoc test.