Table 3.
Brachyury-specific T-cell responses post- vs. pre-vaccination with yeast-brachyury
| Carcinoma | CD4 | CD8 | Dose | # of Responsesa | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Pt # |
CD107a | IFNg | IL2 | TNF | CD107a | IFNg | IL2 | TNF | |||
| Colon | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Colon | 2 | 0 | 0 | 0 | 0 | 48 | 1 | 0 | 68 | 4 YU | 1/3 (33%) |
| Colon | 3 | 432 | 452 | 141 | 41 | 0 | 0 | 0 | 37 | ||
| Colon | 4 | 22 | 0 | 115 | 220 | 0 | 15 | 0 | 20 | ||
| Colon | 5 | 97 | 0 | 208 | 374 | 0 | 0 | 148 | 40 | 16 YU | 1/3 (33%) |
| Colon | 7 | 0 | 135 | 120 | 0 | 175 | 2 | 0 | 119 | ||
| Breast | 8 | 0 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Pancreatic | 9 | 146 | 540 | 345 | 897 | 100 | 0 | 0 | 0 | ||
| Urothelial | 10 | 0 | 0 | 0 | 0 | 0 | 15 | 7 | 0 | ||
| Colon | 11 | 226 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Pancreatic | 12 | 328 | 144 | 19 | 229 | 490 | 0 | 29 | 80 | 40 YU | 4/9 (44%) |
| Pancreatic | 13 | 0 | 0 | 0 | 48 | 0 | 14 | 0 | 0 | ||
| Breast | 14 | 0 | 85 | 356 | 606 | 636 | 420 | 202 | 475 | ||
| Breast | 20 | 0 | 0 | 6 | 0 | 0 | 0 | 131 | 0 | ||
| Colon | 23 | 0 | 0 | 222 | 2363 | 0 | 921 | 0 | 0 | ||
| Breast | 24 | 0 | 0 | 0 | 0 | 0 | 119 | 43 | 0 | ||
| Lung | 25 | 0 | 63 | 227 | 114 | 2728 | 167 | 0 | 519 | ||
| Breast | 27 | 0 | 0 | 0 | 0 | 0 | 3014 | 0 | 215 | 80 YU | 5/6 (83%) |
| Ovarian | 28 | 0 | 0 | 0 | 0 | 0 | 22 | 0 | 446 | ||
| Colon | 29 | 0 | 922 | 1064 | 2977 | 5980 | 2380 | 167 | 7639 | ||
| Colon | 32 | 0 | 58 | 337 | 1557 | 0 | 0 | 822 | 0 | ||
| Chordoma | 15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Chordoma | 16 | 1148 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Chordoma | 17 | 1068 | 652 | 226 | 1067 | 1103 | 390 | 202 | 479 | ||
| Chordoma | 18 | 0 | 68 | 0 | 0 | 0 | 46 | 0 | 0 | 40 YU | 4/7 (57%) |
| Chordoma | 19 | 0 | 191 | 445 | 1178 | 0 | 0 | 0 | 0 | ||
| Chordoma | 21 | 248 | 0 | 0 | 34 | 96 | 0 | 0 | 175 | ||
| Chordoma | 22 | 288 | 321 | 40 | 279 | 120 | 0 | 12 | 186 | ||
| Chordoma | 30 | 1839 | 336 | 0 | 0 | 3627 | 0 | 45 | 0 | ||
| Chordoma | 31 | 0 | 0 | 180 | 0 | 0 | 0 | 0 | 0 | 80 YU | 2/3 (66%) |
| Chordoma | 33 | 0 | 47 | 251 | 0 | 823 | 0 | 0 | 0 | ||
| Chordoma* | 18 | 0 | 679 | 348 | 402 | 1928 | 624 | 346 | 624 | 40 YU | |
a
Cytokine or CD107a in CD4 or CD8.
*
Analyzed on day 288.
Numbers in bold are those positive post- vs. pre-vaccination. Grey rows indicate a patient meeting defined immune response criteria. Absolute # of CD4 of CD8 producing cytokine or CD107a+ / 1×106 cells plated at start of in vitro stimulation.