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. Author manuscript; available in PMC: 2016 Dec 1.

Published in final edited form as: Mol Oral Microbiol. 2015 Jun 19;30(6):474–495. doi: 10.1111/omi.12107

Fig. 4 — Panel A. Transmission experiment outline for 7 deletion mutant strains. SDV = sialoacroadenitis virus. Panel B. Gel photograph showing the presence of each of the deletion strains in the pre-infection pool used to inoculate rat pup jaws (from Day 8; see Panel A). Lane contents are as follows: lane 1: MX804-specific primers (MX0804P7UPFG/ Erm L); lane 2: MU0121-specific primers (MU0121P7UPFG/ Erm L); lane 3: MU0123-specific primers (MU0123P7UPFG/ Erm L); lane 4: MU0124-specific primers (MU0124P7UPFG/ Erm L); lane 5: MU0125-specific primers (MU0125P7UPFG/ Erm L); lane 6: MU1592-specific primers (MU1592P8DNRH/ Erm L); lane 7: MU1450-specific primers (MU1450P7UPFG/ Erm L). Panel C. Representative gel photograph showing amplified DNAs isolated from strains surviving after 19 days in the rat model. The left hemi-jaw from rat #13 (in Group II uninfected pups) was sonicated and DNA prepared for use as a template in a PCR reaction with the gene-specific primers for each of the 7 deletion strains utilized in the experiment. Lane order and primer pairs are as detailed in Panel B (above).

Fig. 4 — Panel A. Transmission experiment outline for 7 deletion mutant strains. SDV = sialoacroadenitis virus. Panel B. Gel photograph showing the presence of each of the deletion strains in the pre-infection pool used to inoculate rat pup jaws (from Day 8; see Panel A). Lane contents are as follows: lane 1: MX804-specific primers (MX0804P7UPFG/ Erm L); lane 2: MU0121-specific primers (MU0121P7UPFG/ Erm L); lane 3: MU0123-specific primers (MU0123P7UPFG/ Erm L); lane 4: MU0124-specific primers (MU0124P7UPFG/ Erm L); lane 5: MU0125-specific primers (MU0125P7UPFG/ Erm L); lane 6: MU1592-specific primers (MU1592P8DNRH/ Erm L); lane 7: MU1450-specific primers (MU1450P7UPFG/ Erm L). Panel C. Representative gel photograph showing amplified DNAs isolated from strains surviving after 19 days in the rat model. The left hemi-jaw from rat #13 (in Group II uninfected pups) was sonicated and DNA prepared for use as a template in a PCR reaction with the gene-specific primers for each of the 7 deletion strains utilized in the experiment. Lane order and primer pairs are as detailed in Panel B (above).