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Histologic assessment of in vitro cell binding of anti-CD15-SPIONs. Prussian blue staining (a, d) and electron microscopy micrographs (b, c, e, f) demonstrate that the positive SPIONs (arrows) are bound to the cell membrane after cells were incubated with anti-CD15-SPIONs (a, b, c), while there is absence of positive SPIONs when cells were incubated with nontargeted SPIONs (d, e, f). Bars in (a) and (d) equal 25 μm; bars in (b), (c), (e), and (f) equal 1 μm.
