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. 2015 Oct 25;2015:430943. doi: 10.1155/2015/430943

Figure 2.

Figure 2

Percentages of Th17 cells were identified according the membrane expression of ROR-γt or intracellular IL-17 in lung adenocarcinoma patients and smoking and nonsmoking control subjects. (a) Distribution of stimulated PBMCs according to forward scatter (FSC) and sideward scatter (SSC) dot plot. A gating was set for CD4+ T-cells. From gated CD4+ T-cells, cells producing IL-17, IFN-γ, or both cytokines were detected. A representative cytometric analysis from a lung adenocarcinoma patient is shown. (b) Percentages and expression levels (measure by iMFI values) of IL-17 from CD4+IL-17+ (Th17) T-cells are shown and compared among the studied groups. The results are reported as mean ± SEM. (c) Distribution of purified CD4+ T-cell in a FSC and SSC dot plot. A gating was set for CD4+ T-cells. From gated CD4+ T-cells, the percentage of ROR-γt+ cells was detected. A representative cytometric analysis from a lung adenocarcinoma patient is shown. (d) Percentages of CD4+ROR-γt+ T-cells from lung adenocarcinoma patients and smoking and nonsmoking control subjects are shown. The results are reported as mean ± SEM.