Figure 2.

Loss of flfl enhances Egr-triggered cell death in the developing eye. Light micrographs of Drosophila eyes ((a)–(e)) or acridine orange staining of eye discs from 3rd instar larvae ((f)–(j)) are shown. Compared with the GMR-Gal4 control ((a) and (f)), GMR > Egr^w induced rough eye phenotype in adulthood (b) and cell death in larval eye disc (g) was not affected by expressing GFP RNAi ((c) and (h)) but was strongly enhanced by expressing flfl RNAi ((e) and (j)). Knocking down flfl alone caused a rough eye phenotype (d) and mild cell death in eye discs (i). Genotypes: GMR-Gal4/+ ((a) and (f)); UAS-Egr^w/+; GMR-Gal4/+ ((b) and (g)); UAS-Egr^w/UAS-GFP-IR; GMR-Gal4/+ ((c) and (h)); UAS-flfl-IR/+; GMR-Gal4/+ ((d) and (i)); UAS-Egr^w/UAS-flfl-IR; GMR-Gal4/+ ((e) and (j)).