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. 2015 Oct 25;2015:671839. doi: 10.1155/2015/671839

Figure 3.

Figure 3

Induction of the RhoA and NF-κB pathways by hCLOCK. (a) Western blot analysis of RhoA pathway effector ROCK1 and NF-κB pathway effectors COX-2, IL-6, and p-P65 in HUVECs transduced with control vector or hCLOCK-overexpressing vector. Expression levels are normalized to β-actin. (b) Western blot analysis showing activated RhoA normalized to total RhoA in HUVECs transduced with control vector or hCLOCK-overexpressing vector. (c) Western blot analysis of the same RhoA and NF-κB pathway effectors in HUVECs transduced with scrambled control vector or shCLOCK vector. (d) Western blot analysis showing activated RhoA normalized to total RhoA in HUVECs transduced with scrambled control vector or shCLOCK vector.