Table 2.
Sequences of the primers and probes used for the CBPV RNA 2 RT-qPCR. Oligonucleotide sequences are located on RNA 2 complete genome of the A-79P isolate (NC_010712.1).
| Equimolar mixture | Sequences | Position on CBPV-RNA 2 (based on A79-P isolate) |
|---|---|---|
| Forward primer mix | AGGCGCCGTAGCTGTTTCT | 590–608 nt1 |
| GCGCCGTGGCTGTTTCT | 592–608 nt | |
|
| ||
| Reverse primer mix | CCCCGATCATATAAGCAAACTTCTC | 637–663 nt |
| CCCGATCATATAGGCAAACTTCTC | 639–662 nt | |
| CCCCGATCATATATGCAAACTTCTC | 639–663 nt | |
|
| ||
| Probe mix | CTGCGGTACTCAGCTCAGCTCGACG | 610–634 nt |
| TGCGGTACTCAGCTCAGCTCGGC | 611–631 nt | |
1nt: nucleotide.