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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2015 Aug 8;87:54–64. doi: 10.1016/j.yjmcc.2015.08.001

Fig. 3. Effect of chronic pacing on Cx43 localization and treatment with Verapamil, L-Type Ca2+ blocker.

Fig. 3

(A) Confocal immunofluorescence assays performed on HL-1 cells revealed that St cells re-localize Cx43 from intercellular appositional membranes to the cytoplasm mainly in the peri-nuclear region. Experiments were repeated three times with similar results. Nuclei were counterstained with Hoescht (blue). Scale bar = 25 μm. (B) (i) Representative images of NS and St HL-1 cells treated with Verapamil (10 μM) for 24 h. (ii) Western Blot analysis of NS and St HL-1 whole cellular lysates treated with 10 μM of Verapamil. Scale bar = 100 μm. Densitometric analysis is reported below (n=5). Data are presented as mean ± s.e.m, One-way ANOVA, followed by Bonferroni’s multiple comparison: * p < 0.05.