A. Effect of “phosphorylation mimetics” of CaVβ2bT164 on CaV1.2 I-V relationship. Ca2+ was the charge carrier and in order to normalize for CaV1.2 channel expression we divided the current amplitude by gating current (Qon)[32]. B. CaVβ2bT164D and CaVβ2bT164A did not alter the voltage dependence ofactivation of CaV1.2 channels, or C. voltage dependence of inactivation of CaV1.2 channels. CaVβ2bWT (n = 8, 8, 4), CaVβ2bT164A (n = 6, 6, 4) and CaVβ2bT164D (n = 9, 9, 6) for panel A, B and C, respectively. Activation (Act.) and inactivation (Inact.).