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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2015 Aug 4;87:152–159. doi: 10.1016/j.yjmcc.2015.07.031

Figure 4. Nifedipine recouples eNOS and restores NO bioavailability in Ang II infused hph-1 mice, and restored DHFR protein level in endothelial cells.

Figure 4

Figure 4

The hph-1 mice were pretreated with 2 different doses of Nifedipine for 2 days (5 or 20 mg/kg/day) prior to being infused with Ang II (0.7 mg/kg per day) for 2 weeks, after which aortas were harvested for electron spin resonance (ESR) determination of superoxide and NO production. A) Aortic eNOS uncoupling activity, indicated by NG-nitro-L-arginine methylester (L-NAME)-sensitive superoxide production. Results shown as the difference between measurements of superoxide production made with and without the addition of L-NAME, where positive values indicate that eNOS is uncoupled **<0.01, n=5–6; B) Aortic NO production determined by ESR. **p<0.01, n=5–8; C) DHFR protein level in endothelial cells as measured with Western blotting. *<0.001 vs all others, n=5 each.