Skip to main content
. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: J Immunol. 2015 Oct 2;195(10):4884–4891. doi: 10.4049/jimmunol.1501194

Figure 4. Increased death rate of lung IM correlates with increased blood monocyte turnover rate in SIV-infected rhesus macaques.

Figure 4

(A) Spearman’s correlation analyses were performed to relate blood monocyte turnover (BrdU) and percent of TUNEL+ IM (cell death) from lungs of uninfected and SIV-infected monkeys collected 24 hr post BrdU injection (n=14), and (B) 48 hr post BrdU injection (n=12). A minimum of 400 cells were counted in at least 5 microscopic fields of lung tissues from SIV-infected (●) and uninfected (★) monkeys. (C) A representative image of IM in the lung tissues from an SIV-infected monkey with high monocyte turnover (55% BrdU-staining monocytes at 24h) is shown. Lung tissues from four uninfected rhesus macaques and ten SIV-infected monkeys with different monocyte turnover rates were stained with anti-CD163 (Green) and anti-BrdU (turnover-Red) antibodies. Cell death/apoptosis was measured by TUNEL (Blue) staining. Images were captured with a Leica TCS SP2 confocal microscope at a final magnification of 1260×.