| Isocratic 95% A, 5% B | 0 – 4.5 min |
| Gradient to 5% A, 95% B | 4.5 – 5.5 min, linear ramp |
| Isocratic 5% A, 95% B | 5.5 – 11 min |
| Gradient to 95% A, 5% B | 11 – 12 min, linear ramp |
| Isocratic 95%A, 5% B (re-equilibration) | 12 – 17 min |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.