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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Mol Neurobiol. 2015 May 8;53(5):2983–2994. doi: 10.1007/s12035-015-9179-1

Figure 3.

Figure 3

Detection of alpha-synuclein-centered radicals in dopaminergic neurons. (A) Confocal images of cryocut midbrain sections from sham + DMPO- and Maneb + Paraquat (MP) + DMPO-treated mice showing the colocalization of anti-DMPO staining with alpha-synuclein and tyrosine hydroxylase. (B) Immunoprecipitation of DMPO adducts. Proteins were immunoprecipitated from nigrostriatal tissue homogenates using either normal mouse IgG as a control (lane1) or with anti-alpha-synuclein antibody followed by Western blot analysis of immunoprecipitates using rabbit anti-DMPO antibody. Blots are representative of four different immunoblots from an equal number of experiments.