Figure 2. Long-term culture of CAIX+ve and CAIX-ve cells.

FACS analysis of CAIX shows that only the CAIX+ve population has the ability to recapitulate the original expression pattern and to reform both populations in long term (6 months) culture A.. The expression levels of CAIX in the long term (6 months) cultures of the sorted cells was confirmed both at RNA B. and protein level C.. Error bars represent the mean ± SD. ** = p < 0.01, n = 3.