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. 2015 Jul 22;6(23):19413–19427. doi: 10.18632/oncotarget.4989

Figure 7. Hypoxic induction of CAIX expression is regulated by chromatin accessibility.

Figure 7

A. Semi-quantitative analysis of chromatin compaction within CA9 genomic locus in CAIX+ve and CAIX-ve cells under normoxic and hypoxic conditions using EpiQ assay ploted againist relative mRNA expression of CA9. B. Levels of tri-methyl H3K9 histone at the CA9 promoter region in CAIX positive and negative cell lines, in normoxia and hypoxia. C. Binding of HIF-1β to the CA9 promoter region in CAIX positive and negative cell lines. D. and E. JQ1 decreases CAIX expression in CAIX+ve and CAIX-ve cells as analysed by FACS and western blot. HIF1α and LDHA proteins levels E. in JQ1 treated CAIX+ve and CAIX-ve cells. Error bars represent the mean ± SD. ***p < 0.001, **p < 0.01, *p < 0.05, n = 3.