Figure 3. BTLA expression on B cell subsets: Mean Fluorescent Intensity (MFI) of BTLA on various B cell subsets at all three time points tested, are shown.

Darker bars/histograms represent the younger cohort, and the lighter bars/histograms represent the aged cohort. Histograms of a representative pair show the MFI intensity on the x-axis and events normalized to Mode on the y axis. Within each cell subset, the first graph shows the MFI with error bars denoting Standard Error of Mean (SEM). The second graph shows the % of cells that were BTLA^hi over the parental population. (*) within the bars indicate statistically significant differences between the two age groups at that time point. (*) above the bars indicate statistically significant differences within the same age group, between different time points as indicated by the lines. P-values were calculated using two way ANOVA, corrected for multiple comparisons using the Holm-Sidak correction.