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. 2015 Jun 17;6(23):19542–19551. doi: 10.18632/oncotarget.4295

Figure 1. The C228T mutation of TERT promoter frequently occurs in BCSCs.

Figure 1

A. BCSCs or NBBCs were sorted with CD44 from bladder tumor tissues or adjacent normal bladder tissues by flow cytometry. B. Cellular morphology of fractionated cells was observed by Giemsa-Wright staining. C. BCSCs and non-BCSCs were stained by anti-CK5 antibody. D. Sphere formation assay shows BCSCs (left panel) displayed significantly enhanced growth capability than non-BCSCs (right panel). E. Single cell genomic DNA was extracted and performed single-cell PCR with specific primers for the TERT promoter. F. Sequence chromatograms of fractionated cells by Sanger DNA sequencing. G. The mutation rates of fractionated cells were presented. **p < 0.01.