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. 2015 Apr 17;6(23):19605–19618. doi: 10.18632/oncotarget.3747

Figure 2. TS alters the expression of EMT markers in normal human bronchial epithelial (NHBE) cells.

Figure 2

A. Cigarette smoke extract (CSE) decreased the protein expression of epithelial markers E-cadherin and ZO-1, and increased protein expression of mesenchymal markers Vimentin and N-cadherin in NHBE cells, as shown by Western blotting. Glyceraldehyde 3-phosphate dehydrogenase was used as loading control. B. CSE decreased E-cadherin protein expression and increased Vimentin protein expression in NHBE cells shown by immunofluorescent staining. C. CSE decreased the expression of E-cadherin and ZO-1 mRNAs, and increased the expression of Vimentin and N-cadherin mRNAs, detected by quantitative reverse transcriptase–polymerase chain reaction after normalization to Glyceraldehyde 3-phosphate dehydrogenase. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, compared with control group.