Skip to main content
. 2015 Apr 17;6(23):19605–19618. doi: 10.18632/oncotarget.3747

Figure 6. ERK5 overexpression attenuates TS-induced alterations in EMT markers' expression in normal human bronchial epithelial (NHBE) cells.

Figure 6

A. Transfection of ERK5 lentiviral vector in NHBE cells ameliorated cigarette smoke extract (CSE)-induced decreases in the protein expression levels of E-cadherin and ZO-1, as well as increases in protein expression levels of Vimentin and N-cadherin, as measured by Western blotting. B. ERK5 overexpression attenuated CSE-reduced E-cadherin protein expression and its increased Vimentin protein expression in NHBE cells, as shown by immunofluorescent staining. C. Overexpression of ERK5 improved CSE-induced alterations in the expression of E-cadherin, ZO-1, Vimentin and N-cadherin mRNAs. LV-control = lentiviral control vector; LV-ERK5 = lentiviral expression vector for ERK5. *P < 0.05, **P < 0.01, compared with control group; #P < 0.05, ##P < 0.01, compared with lentiviral control vector group.