Figure 3. Aggressive Ewing sarcoma cells show membranous CD44 expression and gain increased adherence to the CD44 ligand hyaluronic acid.
A. Cytospin preparations of EW7 cells stained for CD44. Conjugate control is shown in the insert. B. CD44 staining (red) of EW7 cells that are forming vasculogenic networks on Matrigel. Arrows indicate membranous staining. Scale bar represents 100 μm. C. Adhesion capacity of Ewing sarcoma cell lines to hyaluronic acid or D. collagen after 1, 2, and 4 hours. Data in C and D are represented as mean ± SEM (n = 5). *p ≤ 0.05, **p ≤ 0.01.
