Table 1. Functional clustering of highly upregulated genes (≥50-fold) in VM+ (EW7) Ewing sarcoma cells compared VM- (SIM.EW27) Ewing sarcoma cells.
| Functional cluster1 | Gene ontology (GO) | Gene IDs identified in the functional cluster |
|---|---|---|
| Vascular development | GO:0001568 blood vessel development GO:0001944 vasculature development GO:0048514 blood vessel morphogenesis GO:0001525 angiogenesis GO:0016477 cell migration GO:0051674 localization of cell GO:0048870 cell motility |
ANPEP2, CD442, CTGF2, CTHRC1, HTATIP2, IL1B2, IL182, ITGA62, NRP12, PLAU2, S100A2, QKI2, TGM2, THBS12 |
| Coagulation | GO:0042060 wound healing GO:0050817 coagulation GO:0007596 blood coagulation GO:0007599 hemostasis GO:0050878 regulation of body fluid levels |
CD9, CD442, DCBLD2, F2RL2, IL1B2, ITGA22, PLAU2, TFPI22 |
| Cell-matrix interaction | GO:0030155 regulation of adhesion GO:0009611 response to wounding GO:0006928 cell motion GO:0050840 extracellular matrix binding GO:0045785 positive regulation of cell adhesion GO:0005178 integrin binding GO:0043236 laminin binding GO:0007155 cell adhesion GO:0022610 biological adhesion GO:0007160 cell-matrix adhesion GO:0005518 collagen binding GO:0031589 cell-substrate adhesion GO:0032403 protein complex binding GO:0009986 cell surface |
ARHGDIB, CA2, CD9, CD442, CTGF2, DCBLD2, ITGA22, ITGA62, IL1B2, IL182, NRP12, PERP, PTPRR, SPP1, TGFB1, TGM2, THBS12 |
Functional clustering based on DAVID software analysis.
Genes that have been well described for their function in vascular development and/or VM.
Gene IDs: ANPEP (alanyl (membrane) aminopeptidase); ARHGDIB (Rho GDP dissociation inhibitor (GDI) β); CA2 (carbonic anhydrase II); CTGF (connective tissue growth factor); CTHRC1 (collagen triple helix repeat containing 1); DCBLD2 (discoidin, CUB and LCCL domain containing 2); F2RL2 (coagulation factor II (thrombin) receptor-like 2); HTATIP2 (HIV-1 Tat interactive protein 2); ILB1 (interleukin 1β); IL18 (interleukin 18); ITGA2 (integrin α2); ITGA6 (integrin α6); NRP1 (neuropilin 1); PLAU (plasminogen activator, urokinase); PERP (TP53 apoptosis effector); PTPRR (protein tyrosine phosphatase); S100A2 (S100 calcium binding protein A2); SPP1 (secreted phosphoprotein 1); QKI (quaking homolog, KH domain RNA binding); TGFB1 (transforming growth factor β1); TFPI2 (tissue factor pathway inhibitor 2); TGM2 (transglutaminase 2); THBS1 (thrombospondin 1).