Figure 6. KPC1, Jab1, Pirh2, and SKP2 are not involved in COP1-mediated p27 downregulation.
(A) Protein levels of KPC1, Jab1, Pirh2, and SKP2 were not changed when endogenous COP1 expression was inhibited with shRNA (left). Lysates of MDA-MB231 cells infected with either COP1-shRNA or control shRNA were analyzed by IB with the indicated antibodies. Lysates of COP1-overexpressing U2OS stable transfectants and vector control transfectants were analyzed by IB with the indicated antibodies (right). (B) 293T cells were co-transfected with the indicated plasmids. Lysates of cells transfected with indicated plasmids were analyzed by immunoblotting with the indicated antibodies. (C)-(F) 293T cells were co-transfected with the indicated plasmids. Lysates of cells infected with indicated shRNAs were analyzed by immunoblotting with the indicated antibodies.