Figure 1. Sequence analysis and character of TLT-6. (A) Predicted amino acid sequences, domains, and motifs of TLT-6. The leader sequence and transmembrane domain are indicated by dotted lines, and the variable type of immunoglobulin-like domain in the extracellular region is denoted by a solid line. The ITIM-like motifs in the intracellular region of TLT-6 are shown in the box. (B) Analysis of deduced molecular weight for Flag-tagged TLT-6 using an in vitro transcription/translation system. (C) Identification of interacting protein with TLT-6 using co-immunoprecipitation. HeLa cells, endogenously expressing SHIP and SHP-2, were used for transfection with HA-tagged TLT-6, immunoprecipitated with anti-Flag antibody, and then immunoblotted with anti-phospho SHIP or SHP-2 monoclonal antibodies. IB, Immunobloting; IP, immunoprecipitation.
