Figure 3. TLT-6 expressions in hematopoietic stem cells, monocyts and macrophages. (A) Expression patterns of TLT-6 in the surface of hematopoietic lineage cells from the bone marrow of mice analyzed by flow cytometry. Flow cytometry analysis was performed as described in the methods section, on the surface region of CD11b⁺ cells and HSCs (c-Kit⁺Sca-1⁺Lin^-cells) from the bone marrow of mice. Expression of TLT-6 on the surface region of peritoneum (B, C)- and bone marrow (D, E)-derived macrophages. Each isolated macrophage sample was stimulated with LPS (10 ng/ml) during the indicated days. After treatment with anti-TLT-6 monoclonal antibody for 2 days, flow cytometry analysis was carried out as described in the methods section. Numbers in the upper parts of histogram curves indicate the positive intensity values of TLT- 6 expression in cells. Dotted line, control; Solid line, LPS-stimulated condition.