Fig. 2. Effects of inhibitors of MEK and Akt in thrombin-induced expression of CCL11. (A, B) HAoSMCs were incubated with or without U0126 and Akti IV (10 µM each) for 2 h followed by stimulation with thrombin for 9 h. (A) CCL11 transcripts were amplified by RT-PCR (upper panel) and the levels of CCL11 transcripts were determined by realtime PCR (lower panel). (B) The levels of CCL11 protein secreted into culture media were determined by ELISA. Data are expressed as mean±SD (n=3 replicates for each group). ^***p<0.001 vs. control; ^###p<0.001 vs. thrombin.