Fig. 5. Effects of ROS quenchers on expression of CCL11 induced by thrombin. (A, B) HAoSMCs were incubated with or without DPI (10 µM) and NAC (5 mM) for 2 h followed by stimulation with thrombin for 9 h. The levels of CCL11 transcripts were determined by realtime PCR (A), and the levels of CCL11 protein secreted into culture media were determined by ELISA (B). Data are expressed as mean±SD (n=3 replicates for each group). ^***p<0.001 vs. control; ^#p<0.05 vs. thrombin; ^###p<0.001 vs. thrombin.