Analyses of TBC1D9 expression in 8-week-old mouse tissues. (a) Scheme of mouse
TBC1D9 isoforms. Isoform 1 protein (1264 amino acids) possesses two GRAM domains
(dotted box). A TBC domain (solid box) exists in both isoform 1 and isoform 2 (1031
amino acids). The open bars represent regions amplified by RT-PCR. (b) RT-PCR
analysis of Tbc1d9 and Gapdh mRNA in mouse
tissues. Top, amplification of both isoforms of Tbc1d9; bottom,
amplification of Gapdh. (c) In situ hybridization
of Tbc1d9 mRNA in the mouse testis. Light microscopic dark (1, 3)
and light field (2, 4) images of Tbc1d9 mRNA in sections of the
testis. Signal is seen in the cytoplasm of spermatocytes (arrows). (d) and (e)
Immunohistochemical analysis of TBC1D9 proteins in the mouse testis using anti-mouse
TBC1D9 antiserum (d) and anti-human TBC1D9 antibody (e). Top, negative control;
bottom, immunoreactivity is seen in the cytoplasm of spermatocytes (arrows) and
round spermatids (arrowheads). Roman numerals, the stages in the seminiferous
tubules.