Table 1. Primer sequences.
| Gene and type of use | Primer sequences (5’–3’) | Product size (bp) | Position | Reference | |
|---|---|---|---|---|---|
| Forward | Reverse | ||||
| Tbc1d9 isoform 1 and 2, RT-PCR | TCAAGATGAGCCTGACTCCGCC | TACGCACCAGGACTGTGTCCTC | 743 | 3549-4291 (isoform 1) 2850-3592 (isoform 2) |
NM_001111304 (isoform 1) NM_027758 (isoform 2) |
| Gapdh, RT-PCR | AGTCGGAGTGAACGGATTTGG | AGTTGTCATGGATGACCTTGG | 488 | 249-736 | NM_008084 |
| Rab5, cloning for RFP-fusion protein | AAGCTTCGATGGCTAATCGAGGAGC | TCAGTTACTACAACACTGGCTTC | 656 | 468-1115 | NM_025887 |
| Rab7, cloning for RFP-fusion protein | CTCGAGCTATGACCTCTAGGAAGAAAGTG | GTCGACTCAACAACTGCAGCTTTCTGCGG | 638 | 1-624 | NM_009005 |
| Rab9, cloning for RFP-fusion protein | CTCGAGCTATGGCAGGAAAATCGTCTCT | TCAACAGCAAGATGAGTTTG | 614 | 387-992 | NM_019773 |
| Rab11, cloning for RFP-fusion protein | AAGCTTCGATGGGCACCCGCGACGACG | TTAGATGTTCTGACAGCACTGC | 659 | 134-784 | NM_017382 |
| Rab7, site-directed mutagenesis | TGGGACACAGCCGGTCTGGAACGG | CCGTTCCAGACCGGCTGTGTCCCA | (184-207) | NM_009005 | |
| Rab9, site-directed mutagenesis | TGGGACACAGCTGGCCTGGAACGC | GCGTTCCAGGCCAGCTGTGTCCCA | (567-590) | NM_019773 | |
| Rab7, cloning for FLAG-fusion protein | AAGCTTATGACCTCTAGGAAGAAA | GGTACCTCAACAACTGCAGCTTTC | 630 | 1-624 | NM_009005 |
| Rab9, cloning for FLAG-fusion protein | GCGGCCGCGATGGCAGGAAAATCGTCT | GGATCCTCAACAGCAAGATGAGTT | 621 | 387-992 | NM_019773 |
| Rutbc2, cloning for GFP-fusion protein | CTCGAGGCCACCATGGCTTCGGTCCCTGCGGAGGCCGA | AAGCTTCTTGTTCTCAATTAGAATCTGCACC | 3297 | 95-3373 | NM_172718 |