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. 2015 Jun 29;64(4):415–424. doi: 10.1538/expanim.15-0016

Table 3. Co-localization analysis of fluorescent pixels in images from 293T cells expressing TBC-GFP and RFP-Rab mutants.

Transfected genes Co-localization (%)* Non co-localization (%)**
TBC1D9 isoform 1
+ Rab9(Q66L) 39.0 ± 7.3 61.0 ± 7.3
+ Rab7(Q67L) 31.6 ± 5.4 68.4 ± 5.4
TBC1D9 isoform 2
+ Rab9(Q66L) 46.7 ± 7.0 53.3 ± 7.0
+ Rab7(Q67L) 16.4 ± 4.9 83.6 ± 4.9
RUTBC2
+ Rab9(Q66L) 48.3 ± 6.4 51.7 ± 6.4
+ Rab7(Q67L) 1.3 ± 1.0 98.7 ± 1.0

*Percentage of pixels that were both GFP-positive and RFP-positive. **Percentage of pixels that were GFP-positive/RFP-negative or GFP-negative/RFP-positive. P<0.05 vs. respective + Rab9 (Q66L) by unpaired t test with Welch’s correction.