Table 3. Co-localization analysis of fluorescent pixels in images from 293T cells expressing TBC-GFP and RFP-Rab mutants.
| Transfected genes | Co-localization (%)* | Non co-localization (%)** |
|---|---|---|
| TBC1D9 isoform 1 | ||
| + Rab9(Q66L) | 39.0 ± 7.3 | 61.0 ± 7.3 |
| + Rab7(Q67L) | 31.6 ± 5.4 | 68.4 ± 5.4 |
| TBC1D9 isoform 2 | ||
| + Rab9(Q66L) | 46.7 ± 7.0 | 53.3 ± 7.0 |
| + Rab7(Q67L) | 16.4 ± 4.9† | 83.6 ± 4.9 |
| RUTBC2 | ||
| + Rab9(Q66L) | 48.3 ± 6.4 | 51.7 ± 6.4 |
| + Rab7(Q67L) | 1.3 ± 1.0† | 98.7 ± 1.0 |
*Percentage of pixels that were both GFP-positive and RFP-positive. **Percentage of pixels that were GFP-positive/RFP-negative or GFP-negative/RFP-positive. †P<0.05 vs. respective + Rab9 (Q66L) by unpaired t test with Welch’s correction.