Table 3.

Differentially methylated regions and associated genes.

VPA			Manganese			Lead
Gene ID	CpG	VPA : Ctl	Gene ID	CpG	Mn : Ctl	Gene ID	CpG	Pb : Ctl
Col2a1	chr15	−1.25942	Atf1	chr15	−2.40606	Lpo	chr11	−2.1686
Glt25d2	chr1	−1.21282	Lrp5	chr19	−1.53512	Alox5	chr6	−1.94012
App	chr16	−1.15531	Ercc3	chr18	−1.42284	Arhgef10	chr8	−1.61243
Chd7	chr4	−1.11644	Pnpla3	chr15	−1.32399	Pkia	chr3	−1.29882
Ptprz1	chr6	−1.0547	Gpr120	chr19	−1.18607	Frmd4b	chr6	−1.28576
Nf2	chr11	1.022569	Rasgrf2	chr13	−1.13359	Chd7	chr4	−1.14022
Rab3gap1	chr1	1.059968	Chd7	chr4	−1.06245	Derl2	chr11	1.021783
Nmnat2	chr1	1.134621	Hivep2	chr10	1.032248	Ctsf	chr19	1.023935
Eif5a	chr11	1.442196	Ctxn1	chr8	1.063293	Bsn	chr9	1.028517
Pi4k2a	chr19	1.548454	Ovol1	chr19	1.087982	Odz2	chr11	1.066651
R3hdm1	chr1	2.132963	Hk2	chr6	1.132845	Rpp38	chr2	1.205103
			Slc13a5	chr11	1.200217	Mtx2	chr2	1.403068
			Adcy1	chr11	1.272176	Fads3	chr19	1.511005
			Scn5a	chr9	1.381722	Plxna1	chr6	1.563187
			Hs3st3a1	chr11	1.454418	Zbtb5	chr4	1.564412
			Pi4k2a	chr19	1.532898	Dguok	chr6	1.565438
			Dmtf1	chr5	1.597027	Dnm2	chr9	1.584182
			Gdap1	chr1	1.676317	Trib2	chr12	1.654498
			Csmd1	chr8	1.730636	Prpsap2	chr11	1.692807
			Tmeff2	chr1	1.771945	Dst	chr1	2.000012
			Trib2	chr12	1.790036
			Hat1	chr2	1.840714
			Prmt8	chr6	1.858983
			Mrps28	chr3	1.916268
			Tnrc6b	chr15	1.969439

CpG array probe names were associated with gene IDs (UCSC Genome Browser). All array data was normalized to the internal sheered standard and control data was compared to experimental data for differential methylation using log₂⁡ ratios. Reported significant differences were defined by >2-fold change, t-test (p < 0.005). The resulting changes in methylation for exposures were 11 for VPA (5 down, 6 up), 25 for Mn (7 down, 18 up), and 20 for Pb (6 down, 14 up).