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. 2015 Oct 26;2015:974039. doi: 10.1155/2015/974039

Table 1.

The different separation system (see Figure 2).

Chromatograms Column Elution programs
(A) Shim-pack ODS C18 column 0–30 min, isocratic 13% (v/v) acetonitrile
30–55 min, linear gradient 13%–22% (v/v) acetonitrile
55–70 min, linear gradient 22%–40% (v/v) acetonitrile
(B) Merck Purospher STAR C18 column 0–30 min, isocratic 13% (v/v) acetonitrile
30–55 min, linear gradient 13%–22% (v/v) acetonitrile
55–70 min, linear gradient 22%–40% (v/v) acetonitrile
(C) Merck Purospher STAR C18 column 0–30 min, isocratic 18% (v/v) acetonitrile
30–60 min, linear gradient 18%–30% (v/v) acetonitrile
60–70 min, isocratic 30% (v/v) acetonitrile