Figure 2.

Lentiviral vectors effectively infect mouse articular cartilage chondrocytes in vitro. Mouse chondrocytes were isolated from articular cartilage tissues of normal mice and infected with Sox9-encoding lentiviral vector (Lv-Sox9/GFP) or control lentiviral vector-encoding GFP (Lv-GFP) at a multiple of infection (MOI) of 5 for 48 hrs. GFP expressions in the infected cells were visualized under fluorescence microscope (magnification 20 ×). Nuclei were stained with Hochst for identification of the cells. One representative photograph of two treatments is shown.