Figure 4.

Over expression of miR-299-3p promoted H69/ADR cell apoptosis. Cell death is monitored by Annexin V-FITC/PI staining and flow cytometry. Quadrant statistics: early apoptosis cells in lower right (LR), late apoptosis cells in upper right (UR), viable cells in lower left (LL), and in necrosis cells in upper left (UL). A. Flow cytometry analysis of H69/ADR cells transfected with NC or miR-299-3p mimics. B. The percentage of early apoptosis cells, late apoptosis cells, and total apoptosis cells. Data are representative of three experiments, and are expressed as mean ± SD.