Figure 2.

The 5’ untranslated region (UTR) and proximal promoter of FBXW12 are deleted in some of the 30 EOCs. (A) Absence of the FBXW12 5’ UTR region and proximal promoter in eleven of 30 EOCs, as assessed by the lack of amplification of a PCR product targeting a 790 bp genomic fragment upstream of the FBXW12 start codon. (B) Amplification of a 654 bp fragment of the constitutive gene (β-actin) from all tumor samples. Genomic DNA isolated from white cells of healthy women was used as positive control (PC). In all PCR experiments, a reaction with all PCR components with the exception of DNA was used as negative control (NC). PC = positive control, NC = negative control, 1-30 = tumor EOC samples. The diagram on top of panel A depicts the approximate positions of the primers used for amplification.