Figure 4.

FBXW12 proximal promoter is methylated in some cases of EOC. Representative image of the sequencing results from 5 clones containing a 345 bp fragment of the FBXW12 proximal promoter. Sequences were compared against the in silico bisulfite converted fragment of the promoter (second top sequence) by means of the MultiAlin 5.4.1 program. The six CpG methylation sites are indicated in red squares. Three of these sites were identified within recognition sequences for three different transcription factors (C/EBP, Lyf-1 and Oct-1, respectively).