Figure 4.

Intracellular calcium transients mediated by pH 6.0 fast applications due to OGD-Rep and PICK1 knockout. Cells were treated with OGD-Rep and collected for Calcium imaging. A. Two kinds of decay pattern were observed. The upper kind showed gentle decays and stable transient at the fourth acid administration, the lower presented dramatic decays and no stable transient could be attained. B. The amiloride-blockable characteristic and source of calcium transients mediated by acidosis. C. Summary data of alterations of mean calcium transients mediated by acidosis in OGD-Rep neurons in each cell-type. D. Representative traces showing 123% increase in wile-type, 70.7% increase in heterozygote and 68.4% decrease in knockout neurons following OGD-Rep. Data are the mean ± SD in each group. The arrows indicated pH 6.0 application. *P<0.05 vs wild-type control group, #P<0.05 vs control neurons of each group. WT: Wild type cells; HT: Heterozygote cells; KO: PICK1 knockout cells.