Figure 2.

Knockdown of EFEMP2 expression by siRNA transfection. EFEMP2 expression level in five glioma cell lines was analyzed by real-time PCR (A) and Western blot (B). (C, D) RNAi efficiency of EFEMP2 in U87 and U373 cells was analyzed by real-time PCR (C) and Western blot (D). GAPDH was also detected as the control of sample loading. Representative western blots (left panel) and quantitative results (right panel) were shown. siNC: scrambled siRNA transfected cells; siEFEMP2: EFEMP2-siRNA transfected cells (***P<0.001 V.S. siNC).