Figure 5.

Suppressing EFEMP2 expression repressed cell invasion in glioma cells. A, B: Invasion assay of control and EFEMP2 knockdown cells in Matrigel-coated transwell chambers. Cells that invaded into the lower well were stained, photographed and counted under a microscope in five fields (×200). Representative images and quantitative results of cell invasion assay were shown. C, D: Expression of MMP-2 and MMP-9 was evaluated by Western blot. siNC: scrambled siRNA transfected cells; siEFEMP2: EFEMP2-siRNA transfected cells (*P<0.05, **P<0.01, ***P<0.001 V.S. siNC).