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PFOA promoted NF-κB translocation into nuclear from cytosolic. The cytosol and nuclear proteins were isolated from the PFOA-treated DLD-1 cells for Western blotting of p65 (A). After PFOA treatment, the nucleus/cytosol ratio of NF-κB was elevated significantly (B). Meanwhile, immunofluorescence assay showed that green dyes labelled-p65 (indicated by red arrow) accumulated in the blue nuclear (C). (Each experiment was performed independently at least triple times).
