Figure 4.

In vivo tumorigenicity of Gab2-siRNA H1975 and untreated H1975 cells. (A) Representative pictures of tumor masses from mice were taken after 25 days of implanted with H1975 cells or with siRNA-Gab2 H1975 cells. Scale bars: 1 cm (B). Gab2 silencing resulted in significantly H1975 tumor growth inhibition versus mice injected untreated H1975 cells. (C) After surgical excision, significant differences were also evident in tumor weight. (D) There was no significant difference in body weight in the three groups. (E) Total tumor tissue lysates were isolated and Gab2 expression levels were detected by western blotting. #, representatives mice number. (F) Tumor tissues were prepared for immunohistochemistry detection with antibodies against p-PI3K p85^Tyr458 and p-AKT^Thr308, and the p-PI3K p85^Tyr458 and p-AKT^Thr308 positive cells were statistical analyzed. Scale bars: 50 µm (G) Shows western blot of MMP-2/MMP-9 expression and phosphorylated STAT3 in tumor tissues. (Bars are represented as the mean ± SD, n = 6, **P < 0.01, ***P < 0.001 versus mice injected untreated H1975 cells).