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RAC1 level was increased after transfection with RAC1 overexpression plasmid. A. The mRNA level of RAC1 was detected by quantitative real time PCR. The relative mRNA level was calculated using 2-∆∆Ct method. B, C. The protein level of RAC1 was detected by western blot using β-actin as an internal reference. All experiments were repeated three times and the results are presented as mean ± SD. ***P < 0.001.
