Figure 4.

miR-374a negatively regulates Axin2 in OS cell. A. The wild type and mutant complementary sequences of the Axin2 mRNA 3’UTR are shown with the miR-374a sequence. B. luciferase reporter assay showed the inhibitory effect of miR-374a on Axin2 3’UTR luciferase activity in MG63 cells. C. The expression levels of Axin2 protein in MG63 cells transfected with miR-374a mimics and miR-374a inhibitor by Western blot. D. Cell proliferation assay was performed after transfection with si-Axin2 or si-NC. *P < 0.05.