Figure 4.

Snail expression is regulated by GM130. A. Seventy-two hours after transfection with siRNA, total RNA was extracted to examine changes in the mRNA expression levels of the indicated genes. B. The expression of Snail in siRNA-transfected cells was examined by immunoblot. C. 293T cells were cotransfected with the GM130 expression vector together with the pGL4-Snail/promoter and pRK-Luc expression vectors. Three independent experiments were carried out (P < 0.001). D. MKN-45 cells were transfected with GM130 siRNAs together with the pGL4-Snail/promoter and pRK-Luc vectors. After 72 hours, luciferase activity was measured. Three independent experiments were carried out, and the relative luciferase activity is indicated (P < 0.001).