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Expression of HOTTIP was up-regulated in OS. A. The relative expression of HOTTIP was measured by qRT-PCR in 68 OS tissues and paired adjacent non-tumor tissues. The expression of HOTTIP was calculated with the 2-ΔΔCt method using GAPDH levels for normalization. B. Kaplan-Meier plots showed overall survival according to the level of HOTTIP in OS patients who underwent surgery. The data represent the mean ± SD of three independent experiments. *P<0.05.
