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. 2015 Sep 1;8(9):11934–11944.

Figure 3.

Figure 3

Annonaceous acetogenins induced the G2/M phase arrest and regulated cell cycle related proteins in BEL-7402/5-FU and HepG2/ADM cells. A. Cells were treated with 10 µg/ml and 20 µg/ml Annonaceous acetogenins for 24 h. PI staining was used to analyze the cell cycle distribution; B. Histogram of cell cycle distribution of BEL-7402/5-FU and HepG2/ADM. C. Western blot analysis of protein extracts obtained from BEL-7402/5-FU and HepG2/ADM cells; D. mRNA expressions of cyclin D1 in BEL-7402/5-FU and HepG2/ADM cells. Data presented are means ± SD values, N = 3. Bars indicate SD. Significant differences from control were indicated by P<0.05 (*) and P<0.01 (**).